所有質(zhì)粒載體匯總.doc

釀酒酵母表達(dá)載體pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母載體pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF，pY15TEF，pY16TEF,酵母基因重組表達(dá)載體pUG6, pSH47,酵母單雜載體pHISi,pLacZi,pHIS2, pGAD424,酵母雙雜交系統(tǒng):釀酒酵母Y187,釀酒酵母AH109；質(zhì)粒pGADT7,pGBKT7；對(duì)照質(zhì)粒pGBKT7-53，pGBKT7-lam，pGADT7-T，PCL1，釀酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190,畢赤酵母表達(dá)載體pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZA,pAO815,pPIC9k-His,pHIL-S1,pPink hc，配套畢赤酵母Pichiapink,畢赤酵母宿主X33，KM71，KM71H，GS115，原核表達(dá)載體pQE30,31,32,40,60,61,62,等原核表達(dá)載體,包括pET系列，pET-GST，pGEX系列（含GST標(biāo)簽），pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列，pQE系列,pTrc99a,pTrcHis系列，pBV220,221,222,pTXB系列，pLLP-ompA,pIN-III-ompA（分泌型表達(dá)系列）,pQBI63（原核表達(dá)帶熒光）pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF（+）, pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK（+）,pBlueScript SK（-） pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C,大腸桿菌冷激質(zhì)粒：pColdI pColdII pColdIII pColdTF原核共表達(dá)質(zhì)粒：pACYCduet-1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大腸桿菌分子伴侶：pG-KJE8 pGro7 pKJE7 pGTf2 pTf16大腸桿菌宿主細(xì)胞：DH5a JM101 JM103 JM105 JM107 JM109 JM110 Top10 Top10F BL21（DE3） HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21（AI） BL21（DE3）plysS TG1 TB1 DH5a（pir） Tuner（DE3） Bl21 codonplusRIPL Novablue（DE3） Rosetta Rosetta（DE3） Rosetta（DE3）plys Rosetta-gami（DE3） Rosetta-gamiB（DE3）, Rosetta-gamiB（DE3）plysS Orgami（DE3） OrgamiB（DE3） HMS174（DE3）植物表達(dá)/RNAi載體農(nóng)桿菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi載體pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入載體（隨機(jī)突變體庫(kù)）pSKI015,pSKI074,真菌ATMT載體pBIG2RHPH2-GUS-GFP,pBHt1枯草芽孢桿菌表達(dá)載體pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01，配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,廣宿主質(zhì)粒pVLT33RNAi基因沉默干擾敲除載體pSilencer1.0，pSilencer 2.1-U6 hygro，pSilencer 3.1-H1 hygro，pSilencer 3.1-H1 neo，pSilencer 4.1-CMV neo，pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi載體（oligoengine） pSuper-puro RNAi逆轉(zhuǎn)錄病毒載體（clontech）: RNAi-Ready pSIREN-Retro Q，RNAi-Ready pSIREN-RetroQ-ZsGreen（Luciferase shRNA Annealed Oligonucleotide） RNAi慢病毒載體（addgene）: pLKO.1哺乳動(dòng)物表達(dá)載體pcDNA3.1+/-,pcDNA4/HisMax B，pSecTag2 A，pVAX1，pBudCE4.1,pTracer CMV2，pcDNA3.1（-）/myc-His A，pcDNA6-Myc/His B，pCEP4，pIRES，pIRESneo，pIRES hyg3，pCMV-myc，pCMV-HA，pIRES-puro3，pIRES-neo3,pCAGGS哺乳動(dòng)物雙雜交系統(tǒng)pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蛻皮激素誘導(dǎo)系統(tǒng)pIND, pVgRxR,LacSwith II哺乳動(dòng)物誘導(dǎo)表達(dá)系統(tǒng):pOPRSVI，pOPI3CAT，pCMVLacI,GeneSwitch System:pSwitch哺乳動(dòng)物表面展示系統(tǒng):pDisplay,四環(huán)素調(diào)控系統(tǒng)（Invitrogen）：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，pcDNA4/TO/Myc-His/LacZ，pcDNA6/TR四環(huán)素調(diào)控系統(tǒng)（Clontech）：pTet-On，pTet-Off，pTRE2,pRevTRE，pRevTet-On，pRevTet-off信號(hào)通路報(bào)告載體:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1（PMA）-Luc;pGL4.26luc2P/minP/Hygro,pGL4.29luc2P/CRE/Hygro,pGL4.30luc2P/NFAT-RE/Hygro，pGL4.75;p53-Luc，pAP-1-Luc，pNF-B-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系統(tǒng)（invitrogen）pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR，乳酸菌表達(dá)載體及各種乳酸菌乳酸桿菌菌株，pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表達(dá)載體pVE5523，pPG611.1,pPG612.1等和乳酸桿菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii廣宿主表達(dá)載體鏈球菌表達(dá)敲除載體假單胞菌表達(dá)載體pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos載體pLAFR3,pMP2444（GFP）, pHY300PLK,pRT102,pRL1063a,轉(zhuǎn)座子載體pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1，腺病毒載體/慢病毒，逆轉(zhuǎn)錄病毒表達(dá)載體及包裝包膜質(zhì)粒，腺病毒系統(tǒng)（Stratagene）: pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3,配套大腸桿菌BJ5183,293,293T cell line腺相關(guān)病毒系統(tǒng)（Stratagene）：pAAV-MCS，pAAV-RC，pHelper，pAAV-LacZ，pAAV-IRES-hrGFP，pCMV-MCS，慢病毒載體:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry質(zhì)粒載體。病毒包裝：二質(zhì)粒包裝系統(tǒng)一：psPAX2 pMD2.G二質(zhì)粒系統(tǒng)二：pCMV-dR8.2 dvpr pCMV-VSVG三質(zhì)粒包裝系統(tǒng)具有更高的包裝效率：pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包裝系統(tǒng)：PLP1 PLP2 PLP/VSVG基因重組載體敲除載體自殺質(zhì)粒RED重組系統(tǒng)載體pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD（革蘭氏陽(yáng)性菌基因敲除），pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶載體PL253，PL451，PL452，PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374熒光蛋白表達(dá)載體熒光定位質(zhì)粒報(bào)告基因載體pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen，pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1，pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc，pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1，pLEGFP-N1，pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1啟動(dòng)子增強(qiáng)子報(bào)告載體（Promega，帶有Luc熒光報(bào)告基因）：pGL3-basic，pGL3-control，pGL3-enhancer，pGL3-promoter，pRL-TK，pRL-CMV，pRL-SV40,信號(hào)通路報(bào)告載體（Clontech）：pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1（PMA）-Luc信號(hào)通路報(bào)告載體（Promega）：pGL4.26luc2P/minP/Hygro,pGL4.29luc2P/CRE/Hygro,pGL4.30luc2P/NFAT-RE/Hygro，pGL4.75信號(hào)通路報(bào)告載體（Stratagene）：p53-Luc，pAP-1-Luc，pNF-B-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc昆蟲(chóng)表達(dá)載體桿狀病毒表達(dá)載體系統(tǒng)pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大腸桿菌,pMIB v5-His B ,pIZT/V5-His，Taq,Pfu高表達(dá)載體菌株KOD, T4連接酶高表達(dá)質(zhì)粒載體,高產(chǎn)基因工程菌株、酶基因KOD,T4 kinase激酶，Tth反轉(zhuǎn)錄酶MMLV，Klentaq PCR酶，Cre酶，RNA inhibitor抑制酶，DL2000質(zhì)粒BiFC雙分子熒光表達(dá)載體FRET系統(tǒng)載體pBiFC-VC155，pBiFC-VN173，pBiFC-bFosVC155，pBiFC-bFos（deltaZIP）VC155，pBiFC-bjun-VN173，pCE-BiFC-VN173，pCE-BiFC-VC155，真菌表達(dá)載體黑曲霉米曲霉黃曲霉表達(dá)質(zhì)粒pBC-hygro,pAN7-1,pBARGPE1,真菌ATMT農(nóng)桿菌介導(dǎo)轉(zhuǎn)化載體pBHt1,pBIG2RHPH2-GFP-GUS等，酵母單雜交三雜交載體，酵母雙雜交系統(tǒng)pGBKT7，pGADT7，pCL1，pGBKT7-53，pGADT7-T，pGBKT7-Lam，配套釀酒酵母AH109酵母單雜交系統(tǒng)（Clontech）:pHISi,pLacZi,pHis2,pHIS2.1酵母三雜交系統(tǒng):pBridge, YBZ1菌株Cytotrap Two-Hybrid System（Stratagene）: pSos, pSos MAFB, pMyr噬菌體展示系統(tǒng)載體1.噬菌體展示抗體scFv質(zhì)粒：pCANTAB5E宿主菌：TG1輔助噬菌體：M13K07可溶表達(dá)菌：HB2151 2.噬菌體展示抗體Fab質(zhì)粒：pcomb3宿主菌：XL1-Blue輔助噬菌體：VCSM13pEVOL，pBudCE4.1,pBudCE4.1-EGFP,Zeocin,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-miR302/367,pDsRed2-N1,pAcGFP1.1,TALE toolbox kit,pIRES-hyg3,pIRES-Neo3,pIRES-puro3,pSIREN-RetroQ-ZsGreen,GST 14-3-3,pDsRed-Monomer-N1,M50 super8x TOPFlash,pRK793,pCMV-tag5B,pCMV-tag2B，pCMV-N-HA，pUAST,pw-25,PAC-5.1-HISA，pSLIK-Neo,pCMV-myc-cyto,pCMV-myc-nuc,疫苗表達(dá)載體pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ5183,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZ，pORF-TK，pCOX2-Luc,pcDNA3.1（-）-myc-His A,pcDNA3.1-p300,pINP, pOprE, pCR2.1 TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,plenti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRES,tdTomato,FUW-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2 A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-His A,B,C,pcDNA3.1-EGFP,pLEGFP-C1,-MHC Promoter,Marc-145猴腎細(xì)胞，Sf9，BmN昆蟲(chóng)家蠶細(xì)胞，酵母載體pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305，306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L40酵母菌，pESC-His,pYIP5,pHIS2,pHIS2.1,pHISi,pLacZi,酵母重組載體pUG6,pSH47,植物載體pCAMBIA1300-EGFP, pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1，R1601,AGL1農(nóng)桿菌感受態(tài)，原核載體pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami（B）DE3,XL1-Blue,Rosetta gamiB（DE3）pLysS,TKB1,ER2566，DB3.1，JM105,TOP10,DH5a,DH5alpha lambda pir,C43（DE3）菌株感受態(tài)，pTrcHis A,B,C,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,222,pCold I,II,III,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p15A,pET-sumo,pBADHis A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核熒光pQBI63,pMP2444,鏈球菌表達(dá)、敲除載體pKC1139、pOJ260、pWHM3，pSET152，pVA981，多宿主載體pGOAL19,pBBR1MCS-2,-5,-6,pDSK519,pLA2917，pRK2013，pVLT33,pMAD,RN4220金葡菌株，真菌載體pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌載體MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽孢桿菌載體pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽孢桿菌表達(dá)載體pHIS1525,WH320菌,谷氨酸棒狀桿菌表達(dá)載體pEC-XK99E,敲除載體pk18mobsacB,pET載體3a, 3d3c-sumo5b11a A+12a A+15b A+16b A+17b A+19b A+20b A+21a,21b,21d A+22b A+23a,23b A+23a-tev24a K+25b K+26b K+27b K+28a,28b,28c K+28a-sumo28a-p5329a K+30a ,30c K+31b A+32a A+32a pelB A+35b K+39b K+40b K+41a,41b k+42a,42b K+43b A+43.1a A+44a A+48b K+49b K+50b K+pETduet-1pACYCduet-1pCDFduet-1pRSFduet-1pColAduet-1pET HispET DsbApET GSTpET TrxpQE載體PQE2pQE9pQE30,31,32,pQE30UApQE30LacIqpQE40LpQE60pQE70pQE80L其他原核表達(dá)載體PBBR1 MCS Cm+PBBR1 MCS-2 K+PBBR1 MCS-3 Tet+PBBR1 MCS-4 A+PBBR1 MCS-5慶大霉素pMal c2x,p2x,5x,5EpBV220,221,222pTrc99a,pTrcCKS，pTrcHis A,B,CpBAD24,33,43,pBADHis A,B,C,pBADmycHisApEZZ18pThio HisA A+pGEX4T-1,4T-2，4T-3，5X-1,6p-1,2t,3xpColdIpColdIIpColdIIIpColdTFpTwin1pRsetA,B,CPLLP-STQpIN-ompApkk223-3pSE380pJLA502pMBP-C，pMBP-ppTYB11,pCYB1Origene-N-His GST A+pPin point CAT A+pPin point xa1 A+pPin point xa2 A+pPin point xa3 A+pCW ori、常見(jiàn)克隆載體puc18,puc19，puc118，puc119pBlue Script SK+,pBlue ScriptSK+pEGM-3ZF（+） A+pEGM-11ZF（+） A+pACYC184pBR322基因敲除pKD3,pKD4，pKD46,pcp20pK18mobSacB海腎熒光素酶報(bào)告基因pGL3basic，promotor，enhancer，controlpGL3 CMpGL4.29,4.30畢赤酵母表達(dá)載體pPIC9pPIC9kpPIC9k-1pPIC9k-HispPIC3.5pPIC3.5kpAO815pHIL-S1pPICZalphaA，B，CpPICZalpha DpPICZalpha EpPICZalpha GA,GBpPICZalpha FCpPICZA，BpGAPZalphaA，B，CpMETalphaA,B,CpFLD-cat A+pFLD-cat Z+pFLD1 Z+畢赤酵母宿主菌JC220JC308GS200GS190GS115KM71KM71HY1991SMD1168SMD1168HX33釀酒酵母表達(dá)載體pYES2,pYES2-flagpYES2NTA,NTB,NTCpYES3CTpYIP5,pYRP7,pADH2pRSH41,pDC315,pDF15,pUG6pESC-His,BFD14,BFD15,pDNR1,p334,pDR195pRS316,pRS316HAYEplac112,YCplac33,YCplac22-EGFP,YEplac195，YEpLac181,YIP211pYX212，pYX212-VHB釀酒酵母宿主菌INSV-1YPH499YM4271BY4741W303-1A真核哺乳動(dòng)物熒光載體pEGFP N1,N2，N3Origene GFPpLEGFP N1pEGFP C1，C3AIF-EGFP N1pECFP N1,C1pEYFP N1,C1pEYFP-golgipEYFPmempBFPN1FIV-BFPpDsred MonomerN1pDsRed1 mitopDsRed2 mitopDsRed N1，C1pDsRed2 N1,C1pDsRed2-ERmRFP真核哺乳動(dòng)物載體pIRESpIRES2-EGFPpADeasy-1pADeasy-1（Bj5183）pAdtrackpAdtrack CMVpShuttlepShuttle CMVpRL CMVpCMV-HApVAX1pVASTpCIpCI neo+pCMV-mycpflag-CMV2pcDNA3.1 +pcDNA3.1 zeo+pcDNA3.1 neo+pcDNA3.1 MycHisApcDNA3.1 MycHisBpcDNA3.1-HBV genomepcDNA3-mRFPHsp70-pcDNA3.1pcDNA 4 Max Bpsuper GFP neopsuper Retro GFP neopSilencer4.1CMV puro+pSilencer4.1CM neo+psuper GFP neopsuper retro GFP neopdisplaypBud CE4.1ptet on, ptet offpRL-tk，pRL-CMV植物表達(dá)載體pcambia1301,1302,1303,1304,1305.22300,23013300pcambia1301（EHA105）pcambia1381Z,1391ZPBI121，221PBI221-GFP植物農(nóng)桿菌宿主EHA105，LBA4404植物RNAi載體PHANNIBAL，PKANNIBAL啟動(dòng)子檢測(cè)pEGFP-1pkk232-8分子伴侶：PGK