決明子提取物對(duì)急性肝損傷保護(hù)作用的實(shí)驗(yàn)研究

1、決明子提取物對(duì)急性肝損傷保護(hù)作用的實(shí)驗(yàn)研究         【關(guān)鍵詞】  提取物   摘要：目的觀察決明子提取物對(duì)CCl4所致小鼠急性肝損傷的影響。方法60只雄性昆明小鼠隨機(jī)分為6組：正常對(duì)照組、模型對(duì)照組、陽(yáng)性對(duì)照組、大、中、小劑量給藥組。大、中、小劑量給藥組按21.6，10.3，5.1 g/kg灌胃決明子提取物，陽(yáng)性對(duì)照組按0.2 g/kg灌胃聯(lián)苯雙酯，正常對(duì)照組及模型對(duì)照組以等體積生理鹽水灌胃，共7 d。第6天，模型對(duì)照組、陽(yáng)性對(duì)照組和給藥組按2.0 ml/kg背部皮下注射CCl

2、4，正常對(duì)照組背部皮下注射等體積生理鹽水。比較各組血清門冬氨酸氨基轉(zhuǎn)移酶（aspartate transaminase，AST）、丙氨酸氨基轉(zhuǎn)移酶（alanine transaminase，ALT），超氧化物歧化酶(superoxide dismutase，SOD)、丙二醛(malondialdehyde，MDA)，并觀察肝組織形態(tài)學(xué)變化。結(jié)果決明子提取物能顯著降低CCl4所致小鼠血清AST 、ALT含量的升高（P0.01），并能升高SOD的活性，降低MDA含量（P0.05）；減輕對(duì)肝細(xì)胞的病理性損害。結(jié)論決明子提取物對(duì)CCl4所致的小鼠急性肝損傷具有一定的保護(hù)作用。  

3、關(guān)鍵詞：決明子；  急性肝損傷；  保護(hù)作用   Experimental Study on Protective Effect of Semen Cassiae Extract  Against  Acute  Liver  Injury   Abstract：ObjectiveTo observe the effect of Semen Cassiaeextract against carbon tetrachloride induced actute liver injury in mic

4、e. MethodsSixty male mice were randomly divided into normal control group，model control group，bifendate pills control group，high-dosage group，middle- dosage group and low- dosage group. The mice in the above groups were fed for 7 days. The drug groups were pretreated intragastrically with 21.6,10.3,

5、5.1 g/kg extract，bifendate pills groups were pretreated intragastrically with 0.2 g/kg bifendate. Control animals were given the same volumn physiological saline. On the 6th day of feeding, the mice except normal control group were given 2.0 ml/kg carbon tetrachloride by subcutaneous injection. Twen

6、ty-four hours after the intoxication，the serum AST，ALT，SOD activities and MDA content were measured in each group above. ResultsSemen Cassiae extract significantly decreased carbon tetrachloride induced actue hepatic injury with increasing the activities of AST，ALT and the content of MDA ，increased

7、the activities of SOD in serum，the severity of liver injury was slight in the high dosage group in pathological examination. ConclusionIt is revealed that Semen Cassiae extract could prevent carbon tetrachloride from inducing acute liver injury.   Key words：Semen Cassiae extract；  Liv

8、er injury；  Protective effect    決明子Semen Cassiae屬豆科植物決明子成熟干燥種子，處方名為草決明、生決明。決明子具有降血壓、降血脂、導(dǎo)瀉、抗菌、抗血小板聚集等多種作用13。本實(shí)驗(yàn)研究決明子提取物對(duì)肝損傷的保護(hù)作用，并對(duì)其作用機(jī)理進(jìn)行初步探討。   1  材料與方法   1.1  決明子提取物 取500 g生藥材加入4 000 ml（8倍量）60% 乙醇，放置1 h，在60水浴回流2 h，過濾，殘?jiān)尤? 000 ml（6倍量）60% 乙醇60水浴回流2 h，合

9、并濾液，用旋轉(zhuǎn)蒸發(fā)儀回收溶劑，得粗粉59 g（每1 g粗粉相當(dāng)于生藥材8.5 g）。   1.2  試劑聯(lián)苯雙酯滴丸，浙江醫(yī)藥股份有限公司新昌制藥廠生產(chǎn)，批準(zhǔn)文號(hào)：國(guó)藥準(zhǔn)字H33020232；門冬氨酸氨基轉(zhuǎn)移酶（AST）、丙氨酸氨基轉(zhuǎn)移酶（ALT）、超氧化物歧化酶（SOD）、丙二醛（MDA）均購(gòu)自南京建成生物工程研究所。其他試劑為國(guó)產(chǎn)分析純。   1.3  主要儀器722型分光光度計(jì)：中國(guó)廈門分析儀器廠；低溫高速離心機(jī)：美國(guó)Sigma公司；日立全自動(dòng)生化檢測(cè)儀：7600-010型；820型組織切片機(jī)：德國(guó)Leica Instrumen

10、ts公司； OLMPUS CH30型萬能顯微鏡：日本OLMPUS光學(xué)工業(yè)株式會(huì)社。   1.4  實(shí)驗(yàn)動(dòng)物昆明種雄性小鼠，普通級(jí)，體重（20±2）g，由延邊大學(xué)基礎(chǔ)醫(yī)學(xué)院動(dòng)物科提供。   1.5  方法            1.6  統(tǒng)計(jì)學(xué)處理數(shù)據(jù)以均數(shù)±標(biāo)準(zhǔn)差（±s）表示，各組樣本均數(shù)間差別的假設(shè)檢驗(yàn)用方差分析（analysis of variance，ANOVA），P0.05認(rèn)為有統(tǒng)計(jì)學(xué)意義

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