保護堿基列表

1、PCF設計引物時酶切位點的保護酶寡核苷酸序列切割率%2 hr20 hrAcc IGGTCGAC Co0CGGTCGAC CG00CCGGTCGAC CGG00rAfl IIICACATGT G00CCACATGT GG>90>90CCCACATGT GGG>90>90Asc IGGCGCGCC>90>90AGGCGCGCC T>90>90TTGGCGCGCC AA>90>90Ava ICCCCGGG G50>90CCCCCGGG GG>90>90TCCCCCGGG GGA>90>90BamH ICGGAT

2、CC G1025CGGGATCC CG>90>90CGCGGATCC GCG>90>90Bgl IICAGATCT G00GAAGATCT TC75>90GGA AGATCT TCC25>90BssH IIGGCGCGC C00AG GCGCGC CT00TTG GCGCGC CAA50>90BstE IIGGGT(A/T)ACC C010BstX IAACTGCAGAA CCAATGCATTGG00AAAACTGCAG CCAATGCATTGG AA2550CTGCAGAA CCAATGCATTGG ATGCAT25>90Cla ICATCGA

3、T G00GATCGAT C00CCATCGAT GG>90>90CCCATCGAT GGG5050EcoR IGGAATTC CCGGAATTC CGCCGGAATTC CGG>90>90>90>90>90>90Hae IIIGGGGCC CC>90>90AGC GGCC GCT>90>90TTGC GGCC GCAA>90>90Hi nd IIICAAGCTT G00CCAAGCTT GG00CCCAAGCTT GGG1075Kpn IGGGTACC C00GG GGTACC CC>90>90C

4、GGGGTACC CCG>90>90rMlu IGACGCGT C00CGACGCGT CG2550Nco ICCCATGG G00CATG CCATGG CATG5075Nde ICCATATG G00CCCATATG GG00CGCCATATG GCG00GGGTTT CATATG AAACCC00GGAATTC CATATG GAATTCC75>90GGGAATTC CATATG GAATTCCC75>901Nhe IGGCTAGC C00CGGCTAGC CG1025CTAGCTAGC TAG1050Not ITTGCGGCCGC AA00ATTT GCGGCC

5、GC TTTA1010AAATAT GCGGCCGC TATAAA1010ATAAGAAT GCGGCCGC TAAACTAT2590AAGGAAAAAA GCGGCCGC AAAAGGAAAA25>90Nsi ITGC ATGCAT GCA10>90CCAATGCAT TGGTTCTGCAGTT>90>90nPac ITTAATTAA00GTTAATTAA C025CCTTAATTAA GG0>90Pme IGTTTAAAC GGTTTAAAC C GG GTTTAAAC CC AGCTTT GTTTAAAC GGCGCGCCGG0007502550>90

6、Pst IGCTGCAG C00TGCA CTGCAG TGCA1010AA CTGCAG AACCAATGCATTGG>90>90AAAA CTGCAG CCAATGCATTGGAA>90>90CTGCAG AACCAATGCATTGGATGCAT00Pvu ICCGATCG G00ATCGATCG AT1025TCG CGATCG CGA010Sac ICGAGCTC G1010Sac IIGCCGCGG C00TCCCCGCGG GGA50>90Sal IGTCGAC GTCAAAAGGCCATAGCGGCCGC00GCGTCGAC GTCTTGGCCATA

7、GCGGCCGCGG1050ACGC GTCGAC GTCGGCCATAGCGGCCGCGGA A1075Sea IGAGTACT C1025AAA AGTACT TTT7575Sma ICCCGGG010CCCCGGG G010CCCCCGGG GG1050TCCCCCGGG GGA>90>90Spe IGACTAGT C10>90GG ACTAGT CC10>90CGG ACTAGT CCG050CTAG ACTAGT CTAG050Sph IGGCATGC C00CAT GCATGC ATG025ACAT GCATGC ATGT1050Stu IAAGGCCT T

8、>90>90GA AGGCCT TC>90>90AAA AGGCCT TTT>90>90I-Xba ICTCTAGA G00GCTCTAGA GC>90>90TGC TCTAGA GCA75>90CTAG TCTAGA CTAG75>90Xho ICCTCGAG G00CCCTCGAG GG1025CCGCTCGAG CGG1075CCCCGGG G00Xma ICCCCCGGG GG2575CCCCCCGGG GGG50>90TCCCCCCGGG GGGA>90>90注釋：1如果要加在序列的5 '端，就在酶切位點識