甘薯種質(zhì)資源遺傳多樣性的ISSR分析.doc

1、甘薯種質(zhì)資遺傳多樣性的ISSR分析p 打開文本圖片集【摘要】：p ：【目的】利用ISSR分子標(biāo)記分析p 不同類型甘薯種質(zhì)的遺傳多樣性，為甘薯種質(zhì)資的傳播途徑分析p 、分類鑒定、有效利用及雜交親本選擇等提供參考依據(jù)?！痉椒ā繌?00條ISSR引物中篩選出多態(tài)性好、擴(kuò)增條帶清晰且重復(fù)性好的ISSR引物，利用其對129份甘薯種質(zhì)材料進(jìn)行擴(kuò)增，通過DPS 7.05計(jì)算不同種質(zhì)間的遺傳距離，并采用非加權(quán)配對算術(shù)平均法（UPGMA）進(jìn)行聚類分析p ?！窘Y(jié)果】以篩選獲得的20條ISSR引物對129份甘薯種質(zhì)材料進(jìn)行擴(kuò)增，共獲得232條條帶，其中多態(tài)性條帶230條，多態(tài)性條帶比例達(dá)99.14，平均每條ISSR

2、引物擴(kuò)增出11.60條條帶?；贗SSR分子標(biāo)記的5份野生種平均遺傳距離為0.4637，124份甘薯栽培種平均遺傳距離為0.1805。野生種與地方品種、引進(jìn)品種和育成品種間的平均遺傳距離分別為0.4688、0.4618和0.4643;而在124份栽培種中，地方品種與引進(jìn)品種間的平均遺傳距離最大（0.2024），引進(jìn)品種與育成品種間的平均遺傳距離最小（0.1673），地方品種與育成品種間的平均遺傳距離為0.1978。聚類分析p 結(jié)果表明，當(dāng)在遺傳距離為0.320_時(shí)可將野生種與栽培種完全區(qū)分開;5份野生種在遺傳距離為0.320_時(shí)又被劃分為4個(gè)類別;124份栽培種在遺傳距離為0.20_時(shí)可劃分為

3、6個(gè)類別，其中，新種花、福菜薯18號和黃皮9號3個(gè)品種各自單獨(dú)組成一個(gè)類群（第、和類），金山57、豫薯8號和瑞薯1號組成第類;第類包含93個(gè)地方品種和育成品種;第類由25個(gè)地方品種和育成品種組成?！窘Y(jié)論】不同類型和不同來地的甘薯種質(zhì)資間存在較大遺傳差異，以野生種與栽培種間的遺傳差異最大，而栽培種間又以地方品種和引進(jìn)品種間差異較大，即地方品種資在我國甘薯育種親本選擇利用方面還具有很大的應(yīng)用潛力。ISSR分子標(biāo)記是一種適用于甘薯資遺傳多樣性分析p 的理想分子標(biāo)記。【關(guān)鍵詞】：p ： 甘薯;種質(zhì)資;ISSR分子標(biāo)記;遺傳多樣性;聚類分析p 中圖分類號： S531.024 文獻(xiàn)標(biāo)志碼： A 文章編號：

4、2095-1191（20_）11-2392-09Geic diversity analysis of sweetpotato Ipomoea batatas（L.） Lam. by ISSR molecular markersLIU Zhong-hua， LIN Zhi-jian， LI Hua-wei， _U Yong-qing， LI Guo-liang，QIU Yong-_iang， QIU Si-_in_， TANG Hao_（Crops Research Institute， Fujian Academy of Agriculture Sciences/Scientific Obse

5、rving and E_perimental Station of Tuber and Root Crops in South China， Ministry of Agriculture and Rural Affairs ， Fuzhou 350013， China）Abstract：【Objective】In order to provide a theoretical basis for analysis of the domestication pathways， classification and identification， effective utilization and

6、 selection of hybrid parents of sweet potato germplasm， the ISSR molecular markers were used to analyze the geic diversity of different types of sweet potato.【Method】ISSR primers with rich polymorphism， clear lified bands and good repeatability were screened out from 100 primers.The DNA of 129 sweet

7、 potato accessions were lified by the selected ISSR primers， and lified bands were analyzed.The geic distances among various varieties were calculated using DPS 7.05， and the cluster analysis was performed using the un-weighted pair-group arithmetical mean method（UPGMA）.【Result】The results showed th

8、at232 bands were lified by 20 polymorphic ISSR primers selected from 100， of which 230 were polymorphic bands， with a polymorphic ratio of 99.14， and the average bands lified by each primer were 11.60.The average geic distance of five wild species based on ISSR marker was 0.4637， which among 124 swe

9、et potato cultivars was 0.1805.The average geic distance between wild varieties and local varieties， between wild varieties and introduced varieties and wild varieties and bred varieties were 0.4688， 0.4618 and 0.4643.Among all 124 cultivars， the average geic distance between local accessions and th

10、e introduced varieties was the largest（0.2024）， while which between the introduced varieties and the bred varieties was the smallest（0.1673）.The average geic distance between local accessions and bred cultivars was 0.1978.The results of cluster analysis showed that wild species were pletely distingu

11、ished from cultivars when the geic distance was 0.320_.Furthermore， five wild species were divided into four categories at this level， while 124 cultivars were divided into 6 categories at the level of 0.20_.Among them， _inzhonghua， Fucaishu No.18 and Huangpi No.9 were in three single groups（Categor

12、ies I， II， and V） separately， and Jinshan 57， Yushu No.8 and Ruishu No.1 were classified in group III.Meanwhile， Group IV contained 93 local accessions and bred cultivars， and group VI consisted of 25 local accessions and bred cultivars.【Conclusion】There are wide geic differences between different types and different sources of sweet potato germplasm.The geic difference between wild species and cultivars is the largest.Among cultivars， the differences between local accessions and introduced cultivars are larger than others.That means local accessions have great lication potential for